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KMID : 0380019940090010008
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Korean Journal of Biotechnology and Bioengineering
1994 Volume.9 No. 1 p.8 ~ p.14
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Optimization of Environmental Conditions for Hirudin Production from Recombinant Saccharomyces cerevisiae
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Abstract
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The research has been carried out to optimize a recombinant S. cerevisiae fermentation process for the production of an anticoagulant hirudin. The structural gene coding for hirudin was combined with the GAL10 promoter for controlled expression, the MFal signal sequence for hirudin secretion, and the GAL7 terminator for transcriptional termination. Growth medium composition and environmental conditions were optimized for maximizing cell growth and final hirudin concentration. The optimized conditions included yeast extract 40g´f , casamino acid 5g´f , glucose 20g/l . galactose 30g/f , DO 50 % and temperature 30 C. These conditions yielded the specific cell growth rate of 0.13 hr;the final cell density of 30 g cell/Q and the final hirudin concentration of 64mg/h in the batch fermentation with a 2.5 jar fermentor.
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